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magnetic activated cell sorting macs skeletal muscle dissociation kit  (Miltenyi Biotec)


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    Miltenyi Biotec magnetic activated cell sorting macs skeletal muscle dissociation kit
    Magnetic Activated Cell Sorting Macs Skeletal Muscle Dissociation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+skeletal+muscle+dissociation+kit/Skeletal+Muscle+Dissociation+Kit%2C+mouse+and+rat/pm42468527-327-41-50
    Average 96 stars, based on 157 article reviews
    magnetic activated cell sorting macs skeletal muscle dissociation kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Single Cell:

    Article Title: Myeloid-specific tristetraprolin mitigates postsurgical incisional pain by suppressing proinflammatory responses
    Article Snippet: .. For flow analysis, single-cell suspensions of skin were prepared using Mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotec, 130-098-305) as per manufacturer’s instruction. .. Immune cells were separated from myelin debris using Percoll (Cytiva, 17,089,101) gradient centrifugation as before [ ].

    Article Title: Degradable cyclic amino alcohol ionizable lipids as vectors for potent influenza mRNA vaccines
    Article Snippet: The protein was conjugated with phycoerythrin (PE) using a PE lightning link conjugation kit (Abcam) according to the manufacturer’s instructions. .. Muscle tissues were dissociated into a single cell suspension using a mouse skeletal muscle dissociation kit (Miltenyi) according to the manufacturer’s instructions. .. Following dissociation, the dissociation mixture was passed through a 70 μm cell strainer (Corning) and debris was removed from the cell suspensions using debris removal solution (Miltenyi) according to the manufacturer’s instructions.

    other:

    Article Title: The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice.
    Article Snippet: Finally, the slices were air dried and mounted using DPX (06522, Sigma-Aldrich).

    Suspension:

    Article Title: Degradable cyclic amino alcohol ionizable lipids as vectors for potent influenza mRNA vaccines
    Article Snippet: The protein was conjugated with phycoerythrin (PE) using a PE lightning link conjugation kit (Abcam) according to the manufacturer’s instructions. .. Muscle tissues were dissociated into a single cell suspension using a mouse skeletal muscle dissociation kit (Miltenyi) according to the manufacturer’s instructions. .. Following dissociation, the dissociation mixture was passed through a 70 μm cell strainer (Corning) and debris was removed from the cell suspensions using debris removal solution (Miltenyi) according to the manufacturer’s instructions.

    Muscles:

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Article Title: The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice
    Article Snippet: Isolation of satellite cells from Poglut1-cKO mouse skeletal muscle was performed as described by the manufacturer with minor modifications (#130-098-305, #130-104-268; Miltenyi Biotec). .. Briefly, forelimb and hindlimb muscles from P4 Poglut1-cKO and sibling controls were dissociated using the mouse Skeletal Muscle Dissociation Kit and the gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Isolation of satellite cells from Poglut1-cKO mouse skeletal muscle was performed as described by the manufacturer with minor modifications (#130-098-305, #130-104-268; Miltenyi Biotec).

    Article Title: The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice
    Article Snippet: Isolation of satellite cells from Poglut1-cKO mouse skeletal muscle was performed as described by the manufacturer with minor modifications (#130-098-305, #130-104-268; Miltenyi Biotec). .. Briefly, forelimb and hindlimb muscles from P4 Poglut1-cKO and sibling controls were dissociated using the mouse Skeletal Muscle Dissociation Kit and the gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, the homogenate was incubated with microbeads conjugated to a cocktail of monoclonal antibodies against no-target cells (Miltenyi Biotec, Bergisch Gladbach, Germany) at 2-8 °C for 20 min.

    Article Title: The YAP1/TAZ-TEAD transcriptional network regulates gene expression at neuromuscular junctions in skeletal muscle fibers
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of ∼3–6 months old adult C57BL/6 wild type, control or knockout mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130–098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium (40% DMEM, 40% Ham's F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/ml)).

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction.
    Article Snippet: .. Tissue culture, culturing of primary muscle cells, transfection, generation of CRISPR/ Cas9 knockout cells Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Magnetic Cell Separation:

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Article Title: The YAP1/TAZ-TEAD transcriptional network regulates gene expression at neuromuscular junctions in skeletal muscle fibers
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of ∼3–6 months old adult C57BL/6 wild type, control or knockout mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130–098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium (40% DMEM, 40% Ham's F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/ml)).

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction.
    Article Snippet: .. Tissue culture, culturing of primary muscle cells, transfection, generation of CRISPR/ Cas9 knockout cells Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Cell Isolation:

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Article Title: The YAP1/TAZ-TEAD transcriptional network regulates gene expression at neuromuscular junctions in skeletal muscle fibers
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of ∼3–6 months old adult C57BL/6 wild type, control or knockout mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130–098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium (40% DMEM, 40% Ham's F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/ml)).

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction.
    Article Snippet: .. Tissue culture, culturing of primary muscle cells, transfection, generation of CRISPR/ Cas9 knockout cells Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Control:

    Article Title: The YAP1/TAZ-TEAD transcriptional network regulates gene expression at neuromuscular junctions in skeletal muscle fibers
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of ∼3–6 months old adult C57BL/6 wild type, control or knockout mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130–098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium (40% DMEM, 40% Ham's F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/ml)).

    Knock-Out:

    Article Title: The YAP1/TAZ-TEAD transcriptional network regulates gene expression at neuromuscular junctions in skeletal muscle fibers
    Article Snippet: .. Primary skeletal muscle satellite cells were prepared from muscles of ∼3–6 months old adult C57BL/6 wild type, control or knockout mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130–098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium (40% DMEM, 40% Ham's F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/ml)).

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction.
    Article Snippet: .. Tissue culture, culturing of primary muscle cells, transfection, generation of CRISPR/ Cas9 knockout cells Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    Transfection:

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction.
    Article Snippet: .. Tissue culture, culturing of primary muscle cells, transfection, generation of CRISPR/ Cas9 knockout cells Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].

    CRISPR:

    Article Title: The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction.
    Article Snippet: .. Tissue culture, culturing of primary muscle cells, transfection, generation of CRISPR/ Cas9 knockout cells Primary skeletal muscle satellite cells were prepared from muscles of 2–3 months old adult C57BL/6 wild type mice using the mouse Skeletal Muscle Dissociation Kit (Miltenyi Biotech, 130-098-305), followed by mouse MACS Satellite Cell Isolation Kit (Miltenyi Biotech, 130-104-268). .. Cells were used for immediate RNA extraction or seeded on Matrigel-coated plates (Thermo Fisher Scientific, CB-40234) in growth medium [40% DMEM, 40% Ham’s F10, 20% FCS, 1% penicillin/streptomycin, and recombinant human fibroblast growth factor (Promega, G507A, 5 ng/mL)].



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